Enhancing cellular uptake and membrane permeability of gallic acid for breast cancer therapy via folate-tagged PEGylated iron oxide nanoparticles has theronastic agent

Abstract Background In an attempt to prove biological activity enhancement upon escalating the cellular uptake response through ligand and carrier-based via nanoframework, gallic acid was chosen be formulated into PLGA-based polymeric nanoparticles with iron oxide as theranostic agent. Results The pre-formulation studies like FTIR, DSC, XRD, TGA were carried out, which implies good compatibility between drug polymer. Furthermore, prepared by using a single nanoprecipitation method, optimized Box–Behnken design. This design used optimize acid-loaded PEGylated considering effects of three factors ( X 1 ; lipid, 2 PLGA, 3 drug) on variables Y (EE), (size), (drug release). findings surface plots are attributed nanoparticle. in vitro release followed biphasic profile both tested media, pH 4.8 7.4. desirable physicochemical characteristics involved small particle size considerable stability, attained due anionic nature PLGA. cytotoxicity assay acid, GA/PLGA-IONPs, FA-GA/PLGA-PEGylated-LIONPs evaluated MTT assay, showed inhibition effect MCF-7 cells induce apoptosis. Cellular fluorescence show higher destruction based concentration dependence. Conclusions above results that sustain therapeutic target enhancing permeability lipid delivery system.